The prediction from meta-PPISP sent to your email has the following format. --------------------------------------------------------------------------- rediction by meta-PPISP : metamethod for Protein-Protein Interaction Site Prediction Column 1: AA (Amino Acid code) Column 2: Ch (Chain ID) Column 3: AA# (Amino Acid number) Column 4-7: Prediction scores of cons-PPISP, PINUP, Promate, and meta-PPISP Note: PINUP and Promate scores are scaled by 100 Column 8: Prediction of whether the residue is in an interface (P = Positive; N = Negative; - = Burried and not predicted) (Note: P corresponds to a score > 0.34 in 7th column; user may set a different threshold for positive prediction.) ************************************************************************* A C 1 0.045 0.000 0.491 0.048 N Q C 2 0.012 0.000 0.617 0.076 N V C 3 0.019 0.490 0.727 0.256 N I C 4 0.074 0.740 0.823 0.433 P N C 5 0.005 0.490 0.839 0.242 N . . . ..... ..... ..... ..... . . . . ..... ..... ..... ..... . . . . ..... ..... ..... ..... . . . . ..... ..... ..... ..... . F C 106 0.990 0.240 0.773 0.423 P T C 107 0.748 0.000 0.611 0.215 N K C 108 0.514 0.240 0.580 0.375 P I C 109 0.580 0.740 0.608 0.433 P R C 110 0.008 0.740 0.668 0.493 P ************************************************************************* A pdb file in which meta-PPSIP prediction scores are stored as B-factors can be downloaded at http://pipe.scs.fsu.edu/meta-ppisp/000000001.pdb ************************************************************************* If there is no prediction printed above this asterisks line, you should go back and check your submission input. The structure must be in PDB format and chain IDs of protein must be entered. This is an automated email, please do not reply. Please contact pipe@scs.fsu.edu if you have questions or suggestions. http://pipe.scs.fsu.edu